P POTENTIAL Of BAJAKAH STEM ENDOPHYTIC FUNGI (Spatholobus littoralis Hassk) as ANTIBACTERIA AGAINST Escherichia coli
Keywords:
escherichia coli, endophyte, Spatholobus littoralis HasskAbstract
Endophyte fungi isolated from their host plants produce the same secondary metabolic compounds as those produced by their host plants. So that the metabolic compound is no longer taken from the plant directly but from the endophyte fungus. So that the preservation of the plant, especially the bajakah (Spatholobus littoralis Hassk), is maintained and can be developed as a drug without worrying about the biodiversity of the plant. To determine the antibacterial activity of the steel-stem endophyte fungus (Spatholobus littoralis Hassk) in inhibiting Escherichia coli and to identify the content of secondary metabolites of the steel-stem endophyte fungus. True Experimental research method with Post test Only Control Design where there are two randomly selected groups, namely the negative control group of Escherichia coli and the positive control of chloramphenicol with the experimental group of endophytic fungi. Endophyte fungal supernatants antibacterial activity test using disc diffusion and identification of secondary metabolites using TLC (Thin Layer Chromatography). The results of an endophyte supernatant study can inhibit Escherichia coli bacteria characterized by an average diameter of the endophyte supernatant 24.42±5.28 mm and positive control of chloramphenicol with an average diameter of 27.68±1.95 mm. The endophyte supernatant has the secondary metabolic content of flavonoid. The supernatant of the steel rod endophyte fungus is potentially antibacterial to Escherichia coli bacteria and has flavonoid secondary metabolite content.
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