PROFILE OF CHEMICAL COMPOUNDS AND ANTIOXIDANT ACTIVITY OF PAPAYA LEAF EXTRACT (CARICA PAPAYA L.) LIVING IN PEATLANDS
Keywords:Antioxidant, papaya leaf (Carica Papaya L), DPPH, peatlands
Papaya leaves (Carica Papaya L) are empirically widely used by the community to treat dengue fever, reduce fever, improve digestion, this is because of the content of carpine substances in papaya leaves. Peatlands are soils made from piles of dead and decayed plant remains, so that the carbon content in peat soil is very large. Very low pH acidic peat between 2,7 â€“ 5,0. Carbon content 5 â€“ 80 mg and nitrogen content ranging from 0,10 â€“ 1,97%. Mineral content in the soil will affect the content of secondary metabolites and their pharmalogical activities. One of them is antioxidant activity. The purpose to determine the content of chemical compounds and to determine the antioxidant activity of papaya leaf extract (Carica Papaya L) living on peatlands. This research uses the maceration method using 96% ethanol as a solvent. Soaking for 3x24 hours. The extract obtained was then put in a rotary evaporator to form a thick extract. The extract was then identified for its chemical composition using Thin Layer Chromatography and antioxidant activity was measured using the DPPH method (2.2 diphenyl-1 pikrylhydrazil). The results of the content of chemical compounds in papaya leaves that grow on peat soil include flavonoids, alkaloids, and steroids. Antioxidant testing obtained IC50 results of 20.564 Î¼g/mL. In Conclusion Papaya leaves (Carica Papaya L) living on peatlands have a very strong category of antioxidant activity.
Barchi. (2016) . Potensi lahan gambut, berbagai macam jenis gambut di Indonesia.
Mardiah N, Mulyanto C, Amelia A, dkk. (2017). Penentuan Aktivitas Antioksidan dari Ekstrak Kulit Bawang Merah (Allium ascalonicum L.) dengan metode DPPH. Jurnal Pharmascience, 04(02), 147 â€“ 154.
Molyneux P. (2018). The Use of the stable radical Diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol, 26(2), 211-219.
Nugroho H, Haryoto, Santoso B. (2017). Aktivitas antioksidan fraksi polar ekstrak metanol dari kulit kayu batang Shorea acuminatissima dengan metode DPPH. Jurnal Ilmu Dasar, 8(2), 158-164.
Copyright (c) 2021 Mutia Indah Eriana
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.