Identification of chemical compounds and assay of antioxidant activity of leaf extract of daunba'balik Angin (Alphitonia excelsa) downstream left cantung region from various levels of fractions
Keywords:
Antioxidant, Wind-Turning Ba's leaves (Alphitonia Excelsa), DPPH ( 2, 2 diphenyl-1- picrihydrazil) phytochemical screening.Abstract
Background: The leaves of Ba'balik Angin (Alphitonia Excelsa) are one of the plants used by everyday people to bathe because it can produce foam to remove dirt and dust and has benefits as an antiseptic
Objectives: This study was to determine the chemical compound content of the leaves of babalik angin (Alphitonia excelsa) as well as to determine the antioxidant activity of extracts of the leaves of babalik angin (Alphitonia excelsa) from various levels of fractions
Method : In this study using the maceration extraction method using 96% ethanol as a solvent. Soaking was carried out three times for 24 hours, the results of the thick extract were carried out with an evaporator then tested for secondary metabolites with color reagents and qualitative and quantitative tests with DPPH.
Resulth : The results of the secondary metabolite test found in the leaves of Bacan Wind include flavonoids, alkaloids, terpenoids and saponins. In the IC50 value of the leaf extract of the leaf extract with the fraction of N-hexane, Ethyl acetate, Methanol, the antioxidant activity of the leaf extract was more than 500 mg/ml so it was said to be in the category of inactive antioxidant activity.
Conclusion : Antioxidant, Wind-Turning Ba's leaves (Alphitonia Excelsa), DPPH ( 2,2 diphenyl-1- picrihydrazil) phytochemical screening.
References
Agustina, Wulan., Nurhamidah., and Handayani, Dewi. 2017. Phytochemical Screening and Antioxidant Activity of Several Fractions of Castor Bark (Ricinus communis L.). ALOTROP: Journal of Education and Chemistry [Internet]. 1(2). Tersedia pada https://ejournal.unib.ac.id/index.php/alotropjurnal/article/view/3529. [Accessed 27 July 2021].
Aisyah, Rudiansyah, & Destiarti, L. (2019). Isolation and Characteristics of Flavonoid Compounds from Ethyl Acetate Fraction of Senggani Plant Stem (Melastoma malabathricum L.). Journal of Equatorial Chemistry [Internet]. 8(2). 61-66. Tersedia pada: https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/36937/75676583543. [Accessed October 3, 2020].
Amody, Z., & Anggraeni, K. (2017). Identification of Glycosides in Gebang Roots (Coryphs utan) from Landayya Village, Bantaeng Regency. ISSN Pharmacy Magazine 1829-9008 [Internet]. 14(1). 8-13. Tersedia pada: https://uit.e-journal.id/MFN/article/view/3/2. [Accessed 29 September 2020].
Dodo., S. M. (2016). Collection of Banua Botanical Gardens with medicinal potential.Jakarta.
Dwisari, F., Harlia, & Andi, A. H. (2016). Isolation and Characteristics of Terpenoid Compounds Methanol Extract of Blind-Blind Wood Tree Roots (Excoecaria agallocha L.). JKK [Internet]. 5(3). 25-30. Available on:https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/14909/13131. [Accessed 27 September 2020].
Ergina., Nuryanti, Siti., and Pursitasari, Indarini Dwi. 2014. Qualitative Test of Secondary Metabolic Compounds on Palado Leaves (Agave angustifolia)
Fadhly, E., Kusrini, D., & Fachriyah, E. (2015). Isolation, Identification of Alkaloid Compounds from Rivina huilis L. Leaf and Cytotoxic Test Using BSLT (Brine Shrimp Lethality Test) Method. Journal of Chemical Science and Applications [Internet]. 18(2) 67-72. Available on: https://ejournal.undip.ac.id/index.php/ksa/article/view/18460/12919. [Accessed 28 September 2020].
Harborne, J.B. 1996. Phytochemical Method. Mold II. translated by Kosasih Padma Winata and Iwang Soediro. Bandung: ITB Press [Internet]. 12(2). 153-158. Tersedia pada: https://ejournal.akprind.ac.id/index.php/technoscientia/article/view/2659. [Diakses 27 Juli 2021].
Julianto, T.S. (2019). Phytochemicals : Secondary Metabolite Review and Phytochemical Screening.Yogyakarta : INDONESIAN ISLAMIC UNIVERSITY.
Lade BD, PAtil AS,Paikrao HM,Kale AS, Hire KK.2014. A comprehensive working , principles and applications of thin layer chromatography research journal of P harmaceutical, Biological and Chemical Sciences, 5: 486-503
Molyneux, P. 2004. The Use Of The Stable Free Radikal Diphenylpicrylhidrazyl (DPPH) For Estimating Antioxidant Activity. Journal Science Of Technology.
Mukhriani. (2014). Pharmacognostic Analysis. Makassar STATE ISLAMIC UNIVERSITY (UIN) Alaudin
B, Mutmainnah. (2017). Phytochemical Screening of Secondary Metabolic Compounds From Pomegranate (Punica Granatum L.) Ethanol Extract Using Color Test Method. Pharmaceutical Media [Internet]. 13(2). Available at: https://doi.org/10.32382/mf.v13i2.880. [Accessed October 20, 2020].
Ningsih, I. Y. (2016). Herbal Medicine Science Module: Post-Harvest Handling. Pharmaceutical Biology Section. Faculty of Pharmacy, University of Jember.
Noviyanti, Yuska., & Linda, Asri Mei. (2020) Phytochemical Profile of Secondary Metabolite Compounds Ethanol Extract of Sensaat Flower (Melastoma malabathricum L.). Journal Of Pharmaceutical and Sciences (JPS)[Internet].3(1). 1-6. Available at: https://journal-jps.com/index.php/jps/article/view/34/26. [Accessed 6 November 2020].
Nugrahani, Rizki., Andayani, Yayuk., and Hakim, Aliefman. 2016. Phytochemical Screening of Bean Extract (Phaseolus vulgaris L) in Powder Preparation. JPPA: Journal of Science Education Research [Internet]. 2(1). 96-103. Available at: jppipa.unram.ac.id/index.php/jppipa/article/view/38/38. [Accessed July 20, 2021].
Nugroho,Ningsih. (2017). Info on Medicinal Plant Commodities, Agency for the Study and Development of Trade, Ministry of Trade of the Republic of Indonesia.
Raal, A., Meos, A., Hinrikus, T., Heinamaki, J., et al. 2020. Dragendorff's reagent: Historical perspectives and current status of a versatile reagent introduced over 150 years ago at the University of Dorpat, Tartu, Estonia. Pharmazie 75 [Internet]. 299-306. Available at: https://pubmed.ncbi.nlm.nih.gov/32635970/. [Accessed 24 July 2021]
Sopianti, D. S., & Sary, W. D. (2018). Phytochemical Screening and TLC Profile of Secondary Metabolites from Ruku-Ruku (Ocimum sanctum L) Leaves. SCIENTIA Journal of Pharmacy and Health [Internet]. 8(1). 44-52. Available at: http://dx.doi.org/10.36434/scientia.v8i1.118. [Accessed 27 October 2020].
Tarannum Naz. (2013). Chemical and Biological Studies of Medicinal Plants Used by the Yaegl Aboriginal Community of Australia.
Warsi, A R Sholichah. 2017. Phytochemical Screening and Antioxidant Activity of Ethanolic Extract and Ethyl Acetate from Basil Leaf (Ocimum basilicum L.) by DPPh Radical Scavenging Method. IPCUAD [Internet]. Tersedia pada: DOI: 10.1088/1757-899X/259/1/012008. [Diakses: 26 Juli 2021].World Flora Online, 2013 A Project of the World Flora Online Consortium. Spatholobus Littoralis Hassk [Internet]. http://www.worldfloraonline.org/taxon/wfo-0000207208. [Diakses Tanggal 24 Januari 2021].
Wilson, P. G. & Waterhouse, J. T. 1982. A review of the genus Tristania R. Br. (Myrtaceae): a heterogeneous assemblage of five genera Australian Journal of Botany 30.
Aisyah, Rudiansyah, & Destiarti, L. (2019). Isolation and Characteristics of Flavonoid Compounds from Ethyl Acetate Fraction of Senggani Plant Stem (Melastoma malabathricum L.). Journal of Equatorial Chemistry [Internet]. 8(2). 61-66. Tersedia pada: https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/36937/75676583543. [Accessed October 3, 2020].
Amody, Z., & Anggraeni, K. (2017). Identification of Glycosides in Gebang Roots (Coryphs utan) from Landayya Village, Bantaeng Regency. ISSN Pharmacy Magazine 1829-9008 [Internet]. 14(1). 8-13. Tersedia pada: https://uit.e-journal.id/MFN/article/view/3/2. [Accessed 29 September 2020].
Dodo., S. M. (2016). Collection of Banua Botanical Gardens with medicinal potential.Jakarta.
Dwisari, F., Harlia, & Andi, A. H. (2016). Isolation and Characteristics of Terpenoid Compounds Methanol Extract of Blind-Blind Wood Tree Roots (Excoecaria agallocha L.). JKK [Internet]. 5(3). 25-30. Available on:https://jurnal.untan.ac.id/index.php/jkkmipa/article/view/14909/13131. [Accessed 27 September 2020].
Ergina., Nuryanti, Siti., and Pursitasari, Indarini Dwi. 2014. Qualitative Test of Secondary Metabolic Compounds on Palado Leaves (Agave angustifolia)
Fadhly, E., Kusrini, D., & Fachriyah, E. (2015). Isolation, Identification of Alkaloid Compounds from Rivina huilis L. Leaf and Cytotoxic Test Using BSLT (Brine Shrimp Lethality Test) Method. Journal of Chemical Science and Applications [Internet]. 18(2) 67-72. Available on: https://ejournal.undip.ac.id/index.php/ksa/article/view/18460/12919. [Accessed 28 September 2020].
Harborne, J.B. 1996. Phytochemical Method. Mold II. translated by Kosasih Padma Winata and Iwang Soediro. Bandung: ITB Press [Internet]. 12(2). 153-158. Tersedia pada: https://ejournal.akprind.ac.id/index.php/technoscientia/article/view/2659. [Diakses 27 Juli 2021].
Julianto, T.S. (2019). Phytochemicals : Secondary Metabolite Review and Phytochemical Screening.Yogyakarta : INDONESIAN ISLAMIC UNIVERSITY.
Lade BD, PAtil AS,Paikrao HM,Kale AS, Hire KK.2014. A comprehensive working , principles and applications of thin layer chromatography research journal of P harmaceutical, Biological and Chemical Sciences, 5: 486-503
Molyneux, P. 2004. The Use Of The Stable Free Radikal Diphenylpicrylhidrazyl (DPPH) For Estimating Antioxidant Activity. Journal Science Of Technology.
Mukhriani. (2014). Pharmacognostic Analysis. Makassar STATE ISLAMIC UNIVERSITY (UIN) Alaudin
B, Mutmainnah. (2017). Phytochemical Screening of Secondary Metabolic Compounds From Pomegranate (Punica Granatum L.) Ethanol Extract Using Color Test Method. Pharmaceutical Media [Internet]. 13(2). Available at: https://doi.org/10.32382/mf.v13i2.880. [Accessed October 20, 2020].
Ningsih, I. Y. (2016). Herbal Medicine Science Module: Post-Harvest Handling. Pharmaceutical Biology Section. Faculty of Pharmacy, University of Jember.
Noviyanti, Yuska., & Linda, Asri Mei. (2020) Phytochemical Profile of Secondary Metabolite Compounds Ethanol Extract of Sensaat Flower (Melastoma malabathricum L.). Journal Of Pharmaceutical and Sciences (JPS)[Internet].3(1). 1-6. Available at: https://journal-jps.com/index.php/jps/article/view/34/26. [Accessed 6 November 2020].
Nugrahani, Rizki., Andayani, Yayuk., and Hakim, Aliefman. 2016. Phytochemical Screening of Bean Extract (Phaseolus vulgaris L) in Powder Preparation. JPPA: Journal of Science Education Research [Internet]. 2(1). 96-103. Available at: jppipa.unram.ac.id/index.php/jppipa/article/view/38/38. [Accessed July 20, 2021].
Nugroho,Ningsih. (2017). Info on Medicinal Plant Commodities, Agency for the Study and Development of Trade, Ministry of Trade of the Republic of Indonesia.
Raal, A., Meos, A., Hinrikus, T., Heinamaki, J., et al. 2020. Dragendorff's reagent: Historical perspectives and current status of a versatile reagent introduced over 150 years ago at the University of Dorpat, Tartu, Estonia. Pharmazie 75 [Internet]. 299-306. Available at: https://pubmed.ncbi.nlm.nih.gov/32635970/. [Accessed 24 July 2021]
Sopianti, D. S., & Sary, W. D. (2018). Phytochemical Screening and TLC Profile of Secondary Metabolites from Ruku-Ruku (Ocimum sanctum L) Leaves. SCIENTIA Journal of Pharmacy and Health [Internet]. 8(1). 44-52. Available at: http://dx.doi.org/10.36434/scientia.v8i1.118. [Accessed 27 October 2020].
Tarannum Naz. (2013). Chemical and Biological Studies of Medicinal Plants Used by the Yaegl Aboriginal Community of Australia.
Warsi, A R Sholichah. 2017. Phytochemical Screening and Antioxidant Activity of Ethanolic Extract and Ethyl Acetate from Basil Leaf (Ocimum basilicum L.) by DPPh Radical Scavenging Method. IPCUAD [Internet]. Tersedia pada: DOI: 10.1088/1757-899X/259/1/012008. [Diakses: 26 Juli 2021].World Flora Online, 2013 A Project of the World Flora Online Consortium. Spatholobus Littoralis Hassk [Internet]. http://www.worldfloraonline.org/taxon/wfo-0000207208. [Diakses Tanggal 24 Januari 2021].
Wilson, P. G. & Waterhouse, J. T. 1982. A review of the genus Tristania R. Br. (Myrtaceae): a heterogeneous assemblage of five genera Australian Journal of Botany 30.
Downloads
Additional Files
Published
2021-10-21
Issue
Section
Articles
License
Copyright (c) 2021 nadila adelia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
