ANTIBACTERIAL ACTIVITY OF BUNGUR (Langerstroemia speciosa (L) PERS) LEAF EXTRACT AGAINST BACTERIA Staphylococcus aureus ATCC 29213 BY DISC DIFFUSION METHOD AND DILUTION METHOD

Authors

  • Raudatul Jannah
  • Dede Mahdiyah
  • Setia Budi

Keywords:

Antibacterial, Bungur leaf extract, Staphylococcus Aureus ATCC 29213.

Abstract

Infection is one of the diseases that is still the biggest health problem worldwide, including in Indonesia. The most common bacteria causing nosocomial infections is Staphylococcus aureus. One of the plants suspected of having antibacterial activity is Bungur (Lagerstroemia speciosa (L) Pers) Plant, Bungur plant has secondary metabolites such as Alkaloids, Flavonoids, Saponins, Steroids, Terpenoids, and Tannins. The extraction process is done by maceration method with the solvent used is 96% ethanol. The antibacterial activity test of Bungur leaf extract using disc diffusion and dilution methods aims to determine whether Bungur leaf extract can inhibit and kill the growth of  Staphylococcus aureus ATCC 29213. The concentrations used in this test are 50%, 75%,100%, positive control used was Ciprofloxacin and the negative control used was the result of standardized bacterial dilution with mc Farland 0.5. The results of the diffusion method test showed that Bungur leaf was able to inhibit bacterial growth in the presence of an inhibition zone at a concentration of 75% (8.11 mm) but the inhibition zone given was still in the weak category and Bungur leaf extract had no killing power against Staphylococcus aureus ATCC 29213 in the method. dilution in the absence of  MBC values. it is necessary to develop research related to the antibacterial potential of the extract of Bungur leaf by testing the effect of the extract of Bungur leaf in inhibiting and killing the growth of the bacteria Staphylococcus aureus ATCC 29213 with an increased concentration.

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Published

2021-10-21