THIN LAYER CHROMATOGRAPHY PROFILE OF SECONDARY METABOLITES IN TAMPALA TRUNK EXTRACT (Spatholobus littoralis hassk)
Keywords:
Thin Layer Chromatography, eluent, mobile phase, , Tampala Bajakah trunk (Spatholobus littoralis hassk).Abstract
Bajakah Tampala Trunk (Spatholobus littoralis hassk) is a plant that is used as a traditional medicine that is useful for the treatment of rheumatic pain, dysentery, and wound medicine in the community empirically or for generations in Central Kalimantan. The purpose of this study was to determine the Thin Layer Chromatography Profile, the mobile phase or the best eluent in the separation of secondary metabolites in the Bajakah Tampala stem extract (Spatholobus littoralis hassk). The research method on making extract of Bajakah Tampala trunk (Spatholobus littoralis hassk) by maceration method, concentrated by rotary evaporator to obtain thick extract, identification by color reaction. Separation of secondary metabolites by TLC method from various ratios of eluent or mobile phase. The result of Bajakah Tampala trunk (Spatholobus littoralis hassk) extract was Positive secondary metabolites with color reactions are alkaloids, flavonoids, saponins, tannins, and terpenoids. The TLC profile showed the presence of mobile phase or the best eluent in the separation of secondary metabolites, namely alkaloids with a ratio of acetate: dichloromethane (8:8). Flavonoids with a ratio of n-hexane: ethyl acetate (8:12). Saponins with a ratio of Chloroform: Methanol: Water (15:5:1). Tannins with a ratio of butanol: acetic acid (8:2). Terpenoids with a ratio of n-hexane: ethyl acetate (12:8). In Conclusion, The best eluent or mobile phase was found in the separation of metabolite compounds from the extract of Bajakah tampala trunk (Spatholobus littoralis hassk) with TLC method.
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