DETERMINATION OF TOTAL FLAVONOID LEVELS FROM THE N-HEXANE FRACTION OF KIRINYUH LEAF EXTRACT (Chromolaena Odorata L) BY UV-VIS SPECTROPHOTOMETRY METHOD
Keywords:(Chromolaena odorata L), N-hexane fraction, UV-VIS Spectrophotometric Method, Total Flavonoid Content.
In Indonesia, the plant Chromolaena odorata L, known as kirinyuh, has two different properties. This plant can act as a weed or nuisance plant and is useful as medicines in wound healing to treat headaches, antihypertensive and anti-inflammatory. This study was to analyze the flavonoid content of the n-Hexane fraction of Kirinyuh Leaf Extract (Chromolaena odorata L.) by using the UV-Vis SpectrophotometricMethod. This study uses qualitative analysis to identify flavonoid compounds while quantitative analysis to determine flavonoid levels uses a spectrophotometric method to obtain the value of absorbance entered into a linear regression equation, namely y = bx + a obtained from a comparison calibration curve and the results are expressed in mg/gram units. and percent. Â The results from the identification of the shinoda color test on kirinyuh leaf extract (chromolaena Odorata L) were positive for red-orange flavonoids. In the Thin Layer Chromatography test In the Thin Layer Chromatography test seen in visible light, UV 254 light and UV 366 nm light were obtained, stain separation was obtained and after evaporation with ammonia was seen a change in yellow stains in visible light and determination of total levels of flavonoids from kirinyuh leaves (chromolaena Odorata L) by 2,6736 mgQE/g or 2,6736 % Conclusions: From this study, it can be concluded that the n-hexane fraction has a flavonoid content of 26,736 mgQE/g or 2,6736 %.
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