SCREENING OF PHOTOCHEMICALS AND ANTIOXIDANT ACTIVITIES OF LIME ROOT EXTRACTS (Citrus Aurantifolia (Cristm.) Swingle) USING DPPH METHOD

Authors

Keywords:

Lime root, Antioxidant, Phytochemical

Abstract

Indonesia is a country that is very rich in natural ingredients, and has natural products that are very beneficial for health. One natural ingredient that are beneficial for health is lime root which is generally used as an herbal medicine. In Tumbang Kunyi , Central Kalimantan , lime root is believed for generations to treat ulcers. Based on this statement, the researcher intends to conduct further research on lime root. To analyze the content of secondary metabolites and antioxidant activity in lime root extract. This type of research is qualitative descriptive observational. Extraction by maceration method. Phytochemical screening with color reagent test and KLT. Antioxidant activity by DPPH method. Extraction of lime root as much as 400 grams with 7 liters of 96% ethanol obtained a thick extract of 14.52 grams. Phytochemical screening results with the color reagent and KLT showed that lime root contains alkaloids, flavonoids, saponins and tannins and antioxidant activity test results are obtained is 24 , 81 ppm. From this study, it can be concluded that secondary metabolites contained in lime root ( Citrus aurantifolia (Cristm.) Swingle) based on phytochemical tests and KLT contain alkaloids, flavonoids, saponins and tannins. Lime root also has a very active antioxidant activity based on the obtained IC50 value of 24 , 81 ppm.

Author Biography

Napa Sukmilawati, UNIVERSITAS SARI MULIA BANJARMASIN

Bachelor of pharmacy, Fakultas Kesehatan

References

Airat, A., Rosemary, B., Dariva, C. G., Galio, A. F., Enejoh, O. S., Ogunyemi, I. O., Bala, M. S., Instruments, G., Saratha, R., Priya, S. V., Thilagavathy, P., RAMLI, S. B., Jalaludin, N. B., Chigondo, M., Chigondo, F., Studi, P., Dokter, P., Kedokteran, F., Ilmu, D. A. N., … Kurshed, N. H. (2015). Total Phenolic Content and Antioxidant Activity of Flavonoids Isolated From Leaves of Selected Citrus Species. South African Journal of Chemical Engineering, 22(3), 277–281.
ES, S. (2014). Skrining Fitokimia Ekstrak Etanol Daun Gatal (Laportea decumana (Roxb.) Wedd). Pharmacy, 11(01), 98–107.
Fajriaty, I., Ih, H., & Setyaningrum, R. (2018). LAPIS TIPIS DARI EKSTRAK ETANOL DAUN BINTANGUR ( Calophyllum soulattri Burm . F .). 7(1), 54–67.
Ikalinus, R., Widyastuti, S., & Eka Setiasih, N. (2015). Skrining Fitokimia Ekstrak Etanol Kulit Batang Kelor (Moringa Oleifera). Indonesia Medicus Veterinus, 4(1), 71–79.
Ismail, I. (2015). Faktor Yang Mempengaruhi Keputusan Masyarakat Memilih Obat Tradisional Di Gampong Lam Ujong. Idea Nursing Journal, 6(1), 7–14.
Jami’ah, S. R., Ifaya, M., Pusmarani, J., & Nurhikma, E. (2018). Uji Aktivitas Antioksidan Ekstrak Metanol Kulit Pisang Raja (Musa Paradisiaca sapientum) Dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil). Jurnal Mandala Pharmacon Indonesia, 4(1), 33–38. https://doi.org/10.35311/jmpi.v4i1.22
Julizan, N. (2019). Validasi Penentuan Aktifitas Antioksidan Dengan Metode Dpph. Kandaga– Media Publikasi Ilmiah Jabatan Fungsional Tenaga Kependidikan, 1(1). https://doi.org/10.24198/kandaga.v1i1.21473
Kesuma, Y. (2015). Antioksidan Alami dan Sintetik.
La, E. O. J., Sawiji, R. T., & Yuliawati, A. N. (2020). Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus polyrhizus). Indonesian Journal of Pharmacy and Natural Product, 03(February), 45–58.
Lauma, S. W., Pangemanan, D. H. C., & Hutagalung, B. S. P. (2014). Uji Efektifitas Perasan Air Jeruk Nipis (Citrus Aurantifolia S) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Secara in Vitro. Pharmacon, 4(4), 9–15. https://doi.org/10.35799/pha.4.2015.10185
Linarwati, M., Fathoni, A., & Minarsih, M. M. (2016). Studi Deskriptif Pelatihan Dan Pengembangan Sumberdaya Manusia Serta Penggunaan Metode Behavioral Event Interview Dalam Merekrut Karyawan Baru Di Bank Mega Cabang Kudus. Journal of Management, 2(2), 1.
Parama, P. W., Sukrama, I. D. M., & Handoko, S. A. (2019). Uji efektifitas antibakteri ekstrak buah jeruk nipis (Citrus aurantifolia) terhadap pertumbuhan Streptococcus mutans in vitro. Bdj, 3(1), 45.
PEMANFAATAN TANAMAN OBAT TRADISIONAL OLEH MASYARAKAT KELURAHAN MERDEKA KECAMATAN KUPANG TIMUR 2016 Jefrin Sambara, Ni Nyoman Yuliani, Maria Yuniati Emerensiana. (2016).
Putri, Z. F. (2010). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sirih (Piper betle L.) terhadap Multiresisten. Universitas Muhammadiyah Surakarta, 4(9), 30.
Ulfah, M., & Sumantri, S. (2014a). UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK KULIT BUAH JERUK NIPIS (Citrus aurantifolia) DENGAN METODE DPPH (1,1-difenil-2- pikrilhidrazil). E-Publikasi Fakultas Farmasi, 11(2), 9–17.
Ulfah, M., & Sumantri, S. (2014b). UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK KULIT BUAH JERUK NIPIS (Citrus aurantifolia) DENGAN METODE DPPH (1,1-difenil-2- pikrilhidrazil). E-Publikasi Fakultas Farmasi, 11(2), 9–17. https://publikasiilmiah.unwahas.ac.id/index.php/Farmasi/article/view/1363
Wardani, I. gusti A. A. K., & Santoso, P. (2017). Akademi Farmasi Saraswati Denpasar, Jalan Kamboja No 11A Denpasar. 3(2), 97–103.
Widyowati, H., Ulfah, M., & Sumantri. (2014). Uji Aktivitas Antioksidan Ekstrak Etanolik Herba Alfalfa (Medicago sativa L.) dengan Metode DPPH (1,1-Diphenyl-2 Picrylhidrazyl). Jurnal Ilmu Farmasi Dan Farmasi Klinik, 11(1), 25–33.
Winariyanthi, P. E. S. K. Y. E. C. N. L. P. Y. (2017). Erna Cahyaningsih Ni Luh Putu Yuni Winariyanthi. Jurnal Ilmiah Medicamento, 3(2), 61–70.

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Published

2021-10-21

Issue

Vol. 1 No. 1 (2021): International Conference on Health Science

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Articles

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