SCREENING OF PHYTOCHEMICALS AND ANTIBACTERIAL ACTIVITY OF DADANGKAK ROOTS EXTRACTS (Hydrolea spinosa L.) AGAINST Streptococcus mutans
Keywords:
Antibacterial, Root Extract, Dadangkak, Diffusion, DilutionAbstract
Background: Infectious disease is the highest problem in the world, especially in developing countries, such as Indonesia. One of the infection causes is the inappropriate use of antibiotics, which lead to resistance. Exploration of dadangak plant (Hydrolea spinosa L.) which was expected has antibacterial effectiveness due to it contains of secondary metabolites in the form of alkaloids, flavonoids, saponins, and tannins.
Objective: To determine the antibacterial activity in the extracts of dadangak root (Hydrolea spnosa L.) against Sterptococcus mutans by disc diffusion and dilution methods to determine the values of MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration).
Methods: Extraction using maceration method and antibacterial activity testing using disc diffusion and dilution methods with concentrations of 20%, 40%, 60%, 80%, and 100%. Data analysis used qualitative and quantitative descriptive. Results: Secondary metabolites produced by dadangakak root extract were alkaloids, flavonoids, tannins, and saponins which have antibacterial activity. antibacterial activity of extract of dadangak root (Hydrolea spinosa L.) against Streptococcus mutans bacteria by diffusion test resulted in an average inhibition zone of 8.22 mm and using the dilution method to observe MIC which was found at a concentration of 40%. Meanwhile, MBC was not found in all concentrations. Conclusion: Dadangkak root extract has secondary metabolites, namely: alkaloids, flavonoids, tannins, and saponins. The antibacterial activity test using the disc diffusion method showed that there was an inhibition zone, the extract of dadangak root (Hydrolea spinosa L.) had a MIC value at a concentration of 40% against Streptococcus mutans bacteria by the dilution method.
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